FITC Monoklonaler Antikörper

FITC Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

FITC

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

3C10A1

Kat-Nr. : 68132-1-PBS

Synonyme

Fluorescein isothiocyanate

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  WB analysis using 68132-1-Ig (same clone as 68132-1-PBS)

  WB result of FITC antibody (68132-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with FITC conjugated protein and un-conjugated protein as negative control. Antibody to TDP-43 as the positive control to target protein. This data was developed using the same antibody clone with 68132-1-PBS in a different storage buffer formulation.

• 

  WB analysis of BSA using 68132-1-Ig (same clone as 68132-1-PBS)

  WB results of FITC antibody (68132-1-Ig, 1:10000) with BSA and FITC conjugated BSA. This data was developed using the same antibody clone with 68132-1-PBS in a different storage buffer formulation.

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  ELISA experiment of FITC conjugated antibody using 68132-1-Ig (same clone as 68132-1-PBS)

  Indirect ELISA and competitive ELISA results show that this antibody is specific to FITC. Indirect ELISA was performed by coating FITC conjugated BSA at 0ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection (red curve, refer to top X-right Y axis). Competitive ELISA was performed similarly except that different concentration of FITC (active group been quenched) was mixed in 3.3ng/mL primary antibody (blue curve, refere to bottom X-left Y axis). This data was developed using the same antibody clone with 68132-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68132-1-PBS bindet in WB, ELISA FITC und zeigt Reaktivität mit FITC

• Getestete Reaktivität: FITC
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: FITC
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This mouse monoclonal antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.