FKBP10/FKBP65 Polyklonaler Antikörper

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• KD/KO Validated

FKBP10/FKBP65 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12172-1-PBS

Synonyme

FKBP10, FKBP65, FKBP 65, FKBP 10, FK506-binding protein 10

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  WB analysis using 12172-1-AP (same clone as 12172-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12172-1-AP (same clone as 12172-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12172-1-AP (same clone as 12172-1-PBS)

  WB result of FKBP10/FKBP65 antibody (12172-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP10/FKBP65 transfected HeLa cells. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12172-1-AP (same clone as 12172-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 12172-1-AP (same clone as 12172-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 12172-1-AP (same clone as 12172-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 12172-1-AP (same clone as 12172-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 12172-1-AP (same clone as 12172-1-PBS)

  IP result of anti-FKBP10/FKBP65 (IP:12172-1-AP, 4ug; Detection:12172-1-AP 1:600) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 12172-1-AP (same clone as 12172-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 12172-1-AP (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 12172-1-AP (same clone as 12172-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 12172-1-AP (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12172-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12172-1-PBS bindet in WB, IHC, IP, Indirect ELISA FKBP10/FKBP65 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FKBP10/FKBP65 fusion protein Ag2814
• Vollständiger Name: FK506 binding protein 10, 65 kDa
• Berechnetes Molekulargewicht: 582 aa, 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC016467
• Gene symbol: FKBP10
• Gene ID (NCBI): 60681
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FKBP10, also known as FKBP65, is a 65 kDa FK506 binding protein that is a member of the FKBP-type peptidyl-prolyl cis/trans isomerase family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone and binding partner of type I collagen. FKBP65 is expressed in developing tissues and re-expressed in adult tissues following injury. As documented, FKBP10 has been detected to play important roles in the initiation and progression of cancers.