FKBP10/FKBP65 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

FKBP10/FKBP65 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

S94

Kat-Nr. : 81567-1-RR

Synonyme

FKBP10, FKBP65, FKBP 65, FKBP 10, FK506-binding protein 10

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  WB analysis using 81567-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81567-1-RR (FKBP10/FKBP65 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 81567-1-RR

  WB result of FKBP10/FKBP65 antibody (81567-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP10/FKBP65 transfected HeLa cells.

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  IHC staining of human ovary tumor using 81567-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81567-1-RR (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 81567-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81567-1-RR (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 81567-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81567-1-RR against Human FKBP10/FKBP65 were performed. The affinity constant is 32.4 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A375-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:11800
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81567-1-RR bindet in WB, IHC, ELISA FKBP10/FKBP65 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FKBP10/FKBP65 fusion protein Ag2814
• Vollständiger Name: FK506 binding protein 10, 65 kDa
• Berechnetes Molekulargewicht: 582 aa, 64 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC016467
• Gene symbol: FKBP10
• Gene ID (NCBI): 60681
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FKBP10, also known as FKBP65, is a 65 kDa FK506 binding protein that is a member of the FKBP-type peptidyl-prolyl cis/trans isomerase family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone and binding partner of type I collagen. FKBP65 is expressed in developing tissues and re-expressed in adult tissues following injury. As documented, FKBP10 has been detected to play important roles in the initiation and progression of cancers.