FKBP1A Rekombinanter Antikörper

FKBP1A Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250923G12

Kat-Nr. : 86249-1-PBS

Synonyme

12 kDa FK506-binding protein, Calstabin-1, FK506-binding protein 1A, FKBP 1A, FKBP1

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  WB analysis using 86249-1-RR (same clone as 86249-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86249-1-RR (FKBP1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86249-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86249-1-RR (same clone as 86249-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86249-1-RR (FKBP1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86249-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02313-2

  The mean FKBP1A concentration was determined to be 26.2 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02313-2

  Sandwich ELISA standard curve of MP02313-2, Human FKBP1A Recombinant Matched Antibody Pair - PBS only. 86249-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0406. 86249-4-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL.

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  Affinity and Kinetic Characterization of 86249-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86249-1-RR against Human FKBP1A were performed. The affinity constant is 0.899 nM.

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Geprüfte Anwendungen

Produktinformation

86249-1-PBS bindet in WB, Sandwich ELISA, Indirect ELISA FKBP1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FKBP1A fusion protein Ag0406
• Vollständiger Name: FK506 binding protein 1A, 12kDa
• Berechnetes Molekulargewicht: 12 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC001925
• Gene symbol: FKBP1A
• Gene ID (NCBI): 2280
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FKBP1A(FK506-binding protein 1A) is also named as FKBP1, FKBP12 and belongs to the FKBP-type PPIase family.It keeps in an inactive conformation TGFBR1, the TGF-beta type I serine/threonine kinase receptor, preventing TGF-beta receptor activation in absence of ligand and catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.