FKBP2 Polyklonaler Antikörper

FKBP2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11700-1-AP

Synonyme

13 kDa FK506 binding protein, 13 kDa FKBP, FK506 binding protein 2, FK506 binding protein 2, 13kDa, FKBP 13, FKBP 2, FKBP13, FKBP2, Immunophilin FKBP13, PPIase, PPIase FKBP2, Rotamase

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse cerebellum using 11700-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 11700-1-AP (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11700-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11700-1-AP (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11700-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11700-1-AP (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 11700-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11700-1-AP (FKBP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11700-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11700-1-AP (FKBP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

11700-1-AP bindet in WB, IHC, ELISA FKBP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FKBP2 fusion protein Ag2255
• Vollständiger Name: FK506 binding protein 2, 13kDa
• Berechnetes Molekulargewicht: 142 aa, 16 kDa
• Beobachtetes Molekulargewicht: 13-16 kDa
• GenBank-Zugangsnummer: BC003384
• Gene symbol: FKBP2
• Gene ID (NCBI): 2286
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FKBP2 is also named as FKBP13 and belongs to the FKBP-type PPIase family. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. FKBP2 has a 21-amino acid signal peptide and appears to be membrane-associated(PMID:1713687). It is localized to the lumen of the endoplasmic reticulum (ER). FKBP12 and FKBP13 are highly similar proteins, of molecular masses 12 kDa and 13 kDa respectively, with approx.43 % amino acid identity. The strong homology between FKBP12 and FKBP13 suggests that they may share similar biological functions, although, apart from rotamase activity, details relating to the function of either protein are scant(PMID:8373365).

Publikationen

Species	Application	Title
human	WB	J Cell Physiol; Multiple roles for peptidylglycine α-amidating monooxygenase in the response to hypoxia.; Authors - Vishwanatha K S Rao
mouse	WB	Endocrinology; Changes in Corticotrope Gene Expression Upon Increased Expression of Peptidylglycine α-Amidating Monooxygenase.; Authors - Richard E Mains