FKBP2 Monoklonaler Antikörper

FKBP2 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H7E8

Kat-Nr. : 66091-1-PBS

Synonyme

FKBP 2, FKBP 13, FK506-binding protein 2, EC:5.2.1.8, 2H7E8

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  WB analysis of human cerebellum using 66091-1-Ig (same clone as 66091-1-PBS)

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66091-1-Ig (same clone as 66091-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 66091-1-Ig (same clone as 66091-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66091-1-Ig (same clone as 66091-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66091-1-Ig (same clone as 66091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66091-1-Ig (FKBP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66091-1-Ig (same clone as 66091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66091-1-Ig (FKBP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66091-1-Ig (same clone as 66091-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug FKBP2 Monoclonal antibody (66091-1-Ig, Clone:2H7E8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66091-1-PBS bindet in WB, IHC, Indirect ELISA FKBP2 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FKBP2 fusion protein Ag19113
• Vollständiger Name: FK506 binding protein 2, 13kDa
• Berechnetes Molekulargewicht: 142 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC003384
• Gene symbol: FKBP2
• Gene ID (NCBI): 2286
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FKBP2 is also named as FKBP13 and belongs to the FKBP-type PPIase family. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. FKBP2 has a 21-amino acid signal peptide and appears to be membrane-associated(PMID:1713687). It is localized to the lumen of the endoplasmic reticulum (ER). FKBP12 and FKBP13 are highly similar proteins, of molecular masses 12 kDa and 13 kDa respectively, with approx.43 % amino acid identity. The strong homology between FKBP12 and FKBP13 suggests that they may share similar biological functions, although, apart from rotamase activity, details relating to the function of either protein are scant(PMID:8373365).