FKBP3 Polyklonaler Antikörper

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• KD/KO Validated

FKBP3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 11755-1-AP

Synonyme

FKBP25, FKBP 3, FKBP 25, FK506-binding protein 3, EC:5.2.1.8

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  WB analysis of HeLa using 11755-1-AP

  WB result of FKBP3 antibody (11755-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP3 transfected HeLa cells.

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  WB analysis of PC-3 using 11755-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11755-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11755-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11755-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 11755-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11755-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FKBP3 antibody (11755-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 11755-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FKBP3 antibody (11755-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, A431-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Lungenkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

11755-1-AP bindet in WB, IHC, IF/ICC, ELISA FKBP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FKBP3 fusion protein Ag2351
• Vollständiger Name: FK506 binding protein 3, 25kDa
• Berechnetes Molekulargewicht: 224 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC020809
• Gene symbol: FKBP3
• Gene ID (NCBI): 2287
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Cell Mol Med; FKBP3 aggravates the malignant phenotype of diffuse large B-cell lymphoma by PARK7-mediated activation of Wnt/β-catenin signalling; Authors - Xiaojing Xing ; KD Validated
human	IHC	BMC Cancer; Construction of the optimization prognostic model based on differentially expressed immune genes of lung adenocarcinoma.; Authors - Yang Zhai