FKBP5 Polyklonaler Antikörper

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• KD/KO Validated

FKBP5 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(21)

Kat-Nr. : 14155-1-AP

Synonyme

FKBP-51, 51 kDa FK506-binding protein, 54 kDa progesterone receptor-associated immunophilin, AIG6, Androgen-regulated protein 6

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  WB analysis of K-562 using 14155-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14155-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14155-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14155-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14155-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 14155-1-AP

  IP result of anti-FKBP5 (IP:14155-1-AP, 4ug; Detection:14155-1-AP 1:4000) with K-562 cells lysate 1800 ug.

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  IHC staining of human colon cancer using 14155-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14155-1-AP (FKBP5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 14155-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14155-1-AP (FKBP5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 14155-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14155-1-AP (FKBP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, Maus-Thymusgewebe
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

14155-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA FKBP5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FKBP5 fusion protein Ag5337
• Vollständiger Name: FK506 binding protein 5
• Berechnetes Molekulargewicht: 457 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC042605
• Gene symbol: FKBP5
• Gene ID (NCBI): 2289
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FKBP5, also known as FKBP51, belongs to the family of immunophilins, FK506 binding proteins (FKBPs). FKBP5 is a negative regulator of GR activity and plays a key role in the termination of the stress response by GRs. FKBP5 limits GR function by decreasing ligand-binding sensitivity, thereby delaying nuclear translocation and reducing GR-dependent transcriptional activity. The known functions of FKBP5 include the regulation of steroid hormone receptors, inhibiting apoptosis in cancer cells, promoting Akt1 dephosphorylation, and inhibiting the Akt1 pathways, to name a few. FKBP5 is widely expressed and the calculated molecular weight is 51 kDa (PMID: 21119664, PMID: 34077736, PMID: 37154033).

Publikationen

Species	Application	Title
human	IF	Cell Stem Cell; Decoding the temporal and regional specification of microglia in the developing human brain.; Authors - Yanxin Li
human	WB	EMBO J; USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51-AKT signaling.; Authors - Kuntian Luo ; KD Validated
mouse	IHC	Nutrients; Kaempferol, a Phytoprogestin, Induces a Subset of Progesterone-Regulated Genes in the Uterus; Authors - Tova M Bergsten
mouse	IHC	Mol Cancer Ther; Cell intrinsic role of COX-2 in pancreatic cancer development.; Authors - Hill Reginald R
mouse	WB	RNA; The co-chaperones Fkbp4/5 control Argonaute2 expression and facilitate RISC assembly.; Authors - Martinez Natalia J NJ
mouse	WB	Biochem Pharmacol; Developmental nicotine exposure elicits multigenerational disequilibria in proBDNF proteolysis and glucocorticoid signaling in the frontal cortices, striata, and hippocampi of adolescent mice.; Authors - Jordan M Buck