FKBP5 Monoklonaler Antikörper

FKBP5 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3B11F3

Kat-Nr. : 67874-1-Ig

Synonyme

51 kDa FK506 binding protein, 51 kDa FKBP, AIG6, Androgen regulated protein 6, FF1 antigen, FK506 binding protein 5, FKBP 5, FKBP 51, FKBP5, FKBP51, FKBP54, HSP90 binding immunophilin, P54, PPIase, PPIase FKBP5, Ptg 10, Rotamase

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Galerie der Validierungsdaten

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  WB analysis using 67874-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of rat testis using 67874-1-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 67874-1-Ig

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67874-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67874-1-Ig (FKBP5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67874-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67874-1-Ig (FKBP5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67874-1-Ig bindet in WB, IHC, ELISA FKBP5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FKBP5 fusion protein Ag5514
• Vollständiger Name: FK506 binding protein 5
• Berechnetes Molekulargewicht: 457 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC042605
• Gene symbol: FKBP5
• Gene ID (NCBI): 2289
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FKBP5(Peptidyl-prolyl cis-trans isomerase FKBP5 ) interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. Unlike the predominant T-cell expression of the mouse protein, FKBP5 is expressed as a 51-kD protein in a variety of tissues but not in brain, colon, or lung(PMID: 9125197).