FKBP5 Rekombinanter Antikörper

FKBP5 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250798G8

Kat-Nr. : 86189-2-PBS

Synonyme

FKBP-51, 51 kDa FK506-binding protein, 54 kDa progesterone receptor-associated immunophilin, AIG6, Androgen-regulated protein 6

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  WB analysis using 86189-2-RR (same clone as 86189-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86189-2-RR (FKBP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86189-2-PBS in a different storage buffer formulation.

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  Sample test of MP02289-1

  The mean FKBP5 concentration was determined to be 473.3 ng/mL in K-562 cell extract based on a 3.00 mg/mL extract load.

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  Cytometric bead array standard curve of MP02289-1

  Cytometric bead array standard curve of MP02289-1, FKBP5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86189-3-PBS. Detection antibody: 86189-2-PBS. Standard: Ag5337. Range: 1.563-200 ng/mL.

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  Affinity and Kinetic Characterization of 86189-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86189-2-RR against Human FKBP5 were performed. The affinity constant is 0.957 nM.

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Geprüfte Anwendungen

Produktinformation

86189-2-PBS bindet in WB, Cytometric bead array, Indirect ELISA, Sample test FKBP5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FKBP5 fusion protein Ag5337
• Vollständiger Name: FK506 binding protein 5
• Berechnetes Molekulargewicht: 457 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC042605
• Gene symbol: FKBP5
• Gene ID (NCBI): 2289
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FKBP5, also known as FKBP51, belongs to the family of immunophilins, FK506 binding proteins (FKBPs). FKBP5 is a negative regulator of GR activity and plays a key role in the termination of the stress response by GRs. FKBP5 limits GR function by decreasing ligand-binding sensitivity, thereby delaying nuclear translocation and reducing GR-dependent transcriptional activity. The known functions of FKBP5 include the regulation of steroid hormone receptors, inhibiting apoptosis in cancer cells, promoting Akt1 dephosphorylation, and inhibiting the Akt1 pathways, to name a few. FKBP5 is widely expressed and the calculated molecular weight is 51 kDa (PMID: 21119664, PMID: 34077736, PMID: 37154033).