FKBP52 Monoklonaler Antikörper

FKBP52 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B5F1

Kat-Nr. : 66040-2-Ig

Synonyme

51 kDa FK506 binding protein, 52 kDa FK506 binding protein, 52 kDa FKBP, 59 kDa immunophilin, FK506 binding protein 4, FK506 binding protein 4, 59kDa, FKBP 4, FKBP 52, FKBP4, FKBP51, FKBP52, FKBP59, HBI, HSP binding immunophilin, Hsp56, Immunophilin FKBP52, p52, p59, PPIase, PPIase FKBP4, Rotamase

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Galerie der Validierungsdaten

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  WB analysis using 66040-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66040-2-Ig (FKBP52 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66040-2-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66040-2-Ig (FKBP52 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66040-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66040-2-Ig (FKBP52 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66040-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66040-2-Ig (FKBP52 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 66040-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using FKBP52 antibody (66040-2-Ig, Clone: 1B5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human breast cancer using 66040-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using FKBP52 antibody (66040-2-Ig, Clone: 1B5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Mammakarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66040-2-Ig bindet in WB, IHC, IF, ELISA FKBP52 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FKBP52 fusion protein Ag18704
• Vollständiger Name: FK506 binding protein 4, 59kDa
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC007924
• Gene symbol: FKBP4
• Gene ID (NCBI): 2288
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FKBP52 (FK506 binding protein 4) is a member of the immunophilin protein family that comprises intracellular protein effectors of immunosuppressive drugs, and it is known as a steroid receptor-associated protein. As a novel regulator of microtubule dynamics, FKBP52 is associated with the motor protein dynein and with the cytoskeleton during mitosis. FKBP52 is highly expressed in CNS regions susceptible to Alzheimer's, and plays a role in modulating toxicity of Abeta peptides. The protein can associate with the Tau function, and may help to decipher and modulate the events involved in Tau-induced neurodegeneration. In addition, FKBP52 is likely to play a role in growth and development of the male genitalia.