FLAD1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FLAD1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 14118-1-AP

Synonyme

FADS, FAD1, FAD synthase region, FAD synthase, FAD pyrophosphorylase

---

• 

  WB analysis of HEK-293 using 14118-1-AP

  WB result of FLAD1 antibody (14118-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-FLAD1 transfected HEK-293 cells.

• 

  WB analysis of K-562 using 14118-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14118-1-AP (FLAD1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14118-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14118-1-AP (FLAD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14118-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14118-1-AP (FADS antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 14118-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14118-1-AP (FADS antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 14118-1-AP

  IP result of anti-FLAD1 (IP:14118-1-AP, 4ug; Detection:14118-1-AP 1:1000) with K-562 cells lysate 2400 ug.

• 

  IF Staining of HepG2 using 14118-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FLAD1 antibody (14118-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, HepG2-Zellen, L02-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

14118-1-AP bindet in WB, IF/ICC, CoIP, ELISA FLAD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FLAD1 fusion protein Ag5267
• Vollständiger Name: FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 446 aa, 49 kDa
• Beobachtetes Molekulargewicht: 50 kDa, 65-70 kDa
• GenBank-Zugangsnummer: BC011378
• Gene symbol: FLAD1
• Gene ID (NCBI): 80308
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FLAD1 is a protein-coding gene for flavin adenine dinucleotide synthetase (FADS), a key enzyme in the FAD biosynthesis process which contains an N-terminal molybdopterin -binding (MPTb) domain and a C-terminal domain (FADS domain) (PMID: 32714079). Alternative splicing of the human FLAD1 gene generates different isoforms of the enzyme FAD synthase. ~60 and ~50 kDa bands correspond to the expected mitochondrial FADS1 and cytosolic FADS2 proteins, respectively (PMID: 29316637).

Publikationen

Species	Application	Title
human	WB,CoIP	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
mouse	WB	Ecotoxicol Environ Saf; Di (2-ethylhexyl) phthalate reduces sperm motility by decreasing sperm tail energy supply; Authors - Xiao-Lu Li