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FLAD1 Monoklonaler Antikörper

FLAD1 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F9A8

Kat-Nr. : 68491-1-PBS

Synonyme

FAD1, FAD synthase region, FAD synthase, FAD pyrophosphorylase, EC:2.7.7.2

Filter:
  • Western Blot (WB) analysis of various lysates using FLAD1 Monoclonal antibody (68491-1-Ig)

    WB analysis using 68491-1-Ig (same clone as 68491-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68491-1-Ig (FLAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68491-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using FLAD1 Monoclonal antibody (68491-1-Ig)

    WB analysis of U2OS using 68491-1-Ig (same clone as 68491-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 68491-1-Ig (FLAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68491-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FLAD1 Monoclonal antibody (68491-1-Ig)

    WB analysis of HEK-293 using 68491-1-Ig (same clone as 68491-1-PBS)

    WB result of FLAD1 antibody (68491-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FLAD1 transfected HEK-293 cells. This data was developed using the same antibody clone with 68491-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68491-1-PBS bindet in WB, Indirect ELISA FLAD1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen FLAD1 fusion protein Ag6845
Vollständiger Name FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae)
Berechnetes Molekulargewicht 446 aa, 49 kDa
Beobachtetes Molekulargewicht50 kDa
GenBank-ZugangsnummerBC011378
Gene symbol FLAD1
Gene ID (NCBI) 80308
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FLAD1 is a protein-coding gene for flavin adenine dinucleotide synthetase (FADS), a key enzyme in the FAD biosynthesis process which contains an N-terminal molybdopterin -binding (MPTb) domain and a C-terminal domain (FADS domain) (PMID: 32714079). Alternative splicing of the human FLAD1 gene generates different isoforms of the enzyme FAD synthase. ~60 and ~50 kDa bands correspond to the expected mitochondrial FADS1 and cytosolic FADS2 proteins, respectively (PMID: 29316637).