FLI1 Rekombinanter Antikörper

FLI1 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250294F12

Kat-Nr. : 85931-1-RR

Synonyme

FLI 1, FLI-1, Friend leukemia virus integration 1, Proto-oncogene Fli-1

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  WB analysis using 85931-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85931-1-RR (FLI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 85931-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 85931-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 85931-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 85931-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEL using 85931-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HEL cells using FLI1 antibody (85931-1-RR, Clone: 250294F12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of Jurkat using 85931-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using FLI1 antibody (85931-1-RR, Clone: 250294F12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85931-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85931-1-RR against Human FLI1 were performed. The affinity constant is 0.314 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HL-60-Zellen, RAW 264.7-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: Mauslungengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEL cells, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85931-1-RR bindet in WB, IHC, IF/ICC, ELISA FLI1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FLI1 fusion protein Ag36169
• Vollständiger Name: Friend leukemia virus integration 1
• Berechnetes Molekulargewicht: 452 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC010115
• Gene symbol: FLI1
• Gene ID (NCBI): 2313
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Friend leukemia virus integration1 (FLI1) that is also termed EWSR2 encodes a sequence-specific transcriptional activator involved in Ewing's sarcoma (EWS), a malignant small, round, blue cell tumour. It is a rare disease in which cancer cells are found in the bone or in soft tissue. The most common areas in which it occurs are the pelvis, the femur, the humerus, the ribs and clavicle. EWS occurs most frequently in teenagers and young adults. FLI1 protein may act as an aberrant transcription factor, but exact mechanisms of oncogenesis remains unknown.