FLNA Monoklonaler Antikörper

FLNA Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

CloneNo.

1G4H3

Kat-Nr. : 67133-1-Ig

Synonyme

ABP 280, ABPX, Actin binding protein 280, Alpha filamin, DKFZp434P031, Filamin 1, Filamin A, FLN, FLN A, FLN1, FLNA, FMD, MNS, NHBP, Non muscle filamin, OPD, OPD1, OPD2

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Galerie der Validierungsdaten

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  WB analysis using 67133-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67133-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67133-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 67133-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67133-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 67133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67133-1-Ig (FLNA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67133-1-Ig (FLNA antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67133-1-Ig (FLNA antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67133-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FLNA antibody (67133-1-Ig, Clone: 1G4H3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 67133-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FLNA antibody (67133-1-Ig, Clone: 1G4H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Skelettmuskelgewebe, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

67133-1-Ig bindet in WB, IHC, IF, ELISA FLNA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FLNA fusion protein Ag23291
• Vollständiger Name: filamin A, alpha (actin binding protein 280)
• Berechnetes Molekulargewicht: 2647 aa, 280 kDa
• Beobachtetes Molekulargewicht: 280 kDa
• GenBank-Zugangsnummer: BC014654
• Gene symbol: FLNA
• Gene ID (NCBI): 2316
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FLNA encodes a 280 kDa widely expressed actin binding protein called filamin A which can crosslinks actin filaments and links actin filaments to membrane glycoproteins to form a three-dimensional network (PMID:29931263). And filamin A could interact with many other proteins, involved in receptor activation, cell migration and adhesion, cell proliferation, inflammation and tumorigenesis, studies have been reported that FLNA overexpressed in multiple types of tumors, suggesting FLNA may involved in cancer aggressiveness(PMID:29100390). What's more, FLNA is an causative gene of periventricular nodular heterotopia (PNH) (PMID: 29062687)

Publikationen

Species	Application	Title
human	WB,IF	Front Cell Dev Biol; NudC L279P Mutation Destabilizes Filamin A by Inhibiting the Hsp90 Chaperoning Pathway and Suppresses Cell Migration.; Authors - Min Liu
human	IF	Hum Mol Genet; Expression and subcellular localization of USH1C/harmonin in human retina provides insights into pathomechanisms and therapy; Authors - Kerstin Nagel-Wolfrum
human	IHC	Mol Med Rep; Filamin A regulates EGFR/ERK/Akt signaling and affects colorectal cancer cell growth and migration.; Authors - Kun Wang
human	WB	Cell Death Dis; NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.; Authors - Wenwen Chen
human	IF	J Exp Clin Cancer Res; Focal Adhesion Kinase (FAK)-Hippo/YAP transduction signaling mediates the stimulatory effects exerted by S100A8/A9-RAGE system in triple-negative breast cancer (TNBC).; Authors - Damiano Cosimo Rigiracciolo
human	WB	Cell Death Dis; FRMD3 inhibits the growth and metastasis of breast cancer through the ubiquitination-mediated degradation of vimentin and subsequent impairment of focal adhesion; Authors - Wenjun Shao