Filamin A Monoklonaler Antikörper

Filamin A Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G4H3

Kat-Nr. : 67133-1-PBS

Synonyme

FLNA, 1G4H3, ABP-280, Actin-binding protein 280, Alpha filamin

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  WB analysis using 67133-1-Ig (same clone as 67133-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67133-1-Ig (Filamin A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67133-1-Ig (same clone as 67133-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67133-1-Ig (Filamin A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67133-1-Ig (same clone as 67133-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67133-1-Ig (Filamin A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67133-1-Ig (same clone as 67133-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67133-1-Ig (Filamin A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 67133-1-Ig (same clone as 67133-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67133-1-Ig (Filamin A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67133-1-Ig (same clone as 67133-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67133-1-Ig (Filamin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67133-1-Ig (same clone as 67133-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67133-1-Ig (Filamin A antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67133-1-Ig (same clone as 67133-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67133-1-Ig (Filamin A antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67133-1-Ig (same clone as 67133-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Filamin A antibody (67133-1-Ig, Clone: 1G4H3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67133-1-Ig (same clone as 67133-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Filamin A antibody (67133-1-Ig, Clone: 1G4H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67133-1-PBS bindet in WB, IHC, IF/ICC, ELISA Filamin A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Filamin A fusion protein Ag23291
• Vollständiger Name: filamin A, alpha (actin binding protein 280)
• Berechnetes Molekulargewicht: 2647 aa, 280 kDa
• Beobachtetes Molekulargewicht: 280 kDa
• GenBank-Zugangsnummer: BC014654
• Gene symbol: FLNA
• Gene ID (NCBI): 2316
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FLNA encodes a 280 kDa widely expressed actin binding protein called filamin A which can crosslinks actin filaments and links actin filaments to membrane glycoproteins to form a three-dimensional network (PMID:29931263). And filamin A could interact with many other proteins, involved in receptor activation, cell migration and adhesion, cell proliferation, inflammation and tumorigenesis, studies have been reported that FLNA overexpressed in multiple types of tumors, suggesting FLNA may involved in cancer aggressiveness(PMID:29100390). What's more, FLNA is an causative gene of periventricular nodular heterotopia (PNH) (PMID: 29062687)