Filamin B Polyklonaler Antikörper

Filamin B Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 20685-1-AP

Synonyme

FLNB, FilaminB, Filamin-3, Beta-filamin, Actin-binding-like protein

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  WB analysis using 20685-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20685-1-AP (Filamin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 20685-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 20685-1-AP (FLNB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 20685-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20685-1-AP (FLNB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 20685-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FLNB antibody (20685-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C2C12-Zellen, NIH/3T3-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

20685-1-AP bindet in WB, IHC, IF/ICC, ELISA Filamin B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: filamin B, beta (actin binding protein 278)
• Berechnetes Molekulargewicht: 278 kDa
• Beobachtetes Molekulargewicht: 250-278 kDa
• GenBank-Zugangsnummer: NM_001457
• Gene symbol: FLNB
• Gene ID (NCBI): 2317
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FLNB, also named as FLN1L, FLN3, TABP, TAP and Fh1, belongs to the filamin family. FLNB connects cell membrane constituents to the actin cytoskeleton. It may promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. It is an anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA, FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. The antibody is specific to FLNB. It can recognize isoform1,2,3,6,7 of FLNB. It has no cross reaction to FLNA and FLNC.

Publikationen

Species	Application	Title
mouse	IHC	J Hazard Mater; Insight into perfluorooctanoic acid-induced impairment of mouse embryo implantation via single-cell RNA-seq; Authors - Xiaoqiang Sheng
mouse	WB	Heliyon; Filamin B knockdown impairs differentiation and function in mouse pre-osteoblasts via aberrant transcription and alternative splicing; Authors - Xi Wang ; KD Validated
human	WB	Cell Death Dis; NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.; Authors - Wenwen Chen
mouse	IHC	J Genet Genomics; Rare loss-of-function variants in FLNB cause non-syndromic orofacial clefts; Authors - Wenbin Huang
human,mouse	IHC,IF	Am J Cancer Res; FLNB overexpression promotes tumor progression and associates with immune suppression, evasion and stemness in pancreatic cancer; Authors - Guoyong Fan