Flotillin 1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Flotillin 1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(54)

Rezensionen (1)

Kat-Nr. : 15571-1-AP

Synonyme

FLOT1, Flotillin-1

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• 

  WB analysis using 15571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15571-1-AP (Flotillin 1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15571-1-AP (Flotillin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15571-1-AP (Flotillin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15571-1-AP

  IP result of anti-Flotillin 1 (IP:15571-1-AP, 4ug; Detection:15571-1-AP 1:500) with mouse brain tissue lysate 4400ug.

• 

  IHC staining of human stomach using 15571-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15571-1-AP (Flotillin 1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15571-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Flotillin 1 antibody (15571-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausherzgewebe, Rattenhirngewebe, Rattenherzgewebe, U-251-Zellen, U-87 MG-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 51 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Produktinformation

15571-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA Flotillin 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Flotillin 1 fusion protein Ag7902
• Vollständiger Name: flotillin 1
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC001146
• Gene symbol: Flotillin 1
• Gene ID (NCBI): 10211
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

Publikationen

Species	Application	Title
human	WB	J Extracell Vesicles; Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.; Authors - Xiaohan Gao
human	WB	Brain; Extracellular vesicle biomarkers for complement dysfunction in schizophrenia; Authors - Ting Xue
mouse,human	WB	Theranostics; Microenvironment-induced CREPT expression by cancer-derived small extracellular vesicles primes field cancerization; Authors - Yuting Lin
mouse	WB	Cell Res; Translocation of mixed lineage kinase domain-like protein to plasma membrane leads to necrotic cell death.; Authors - Xin Chen
mouse,human	WB,IHC	Int J Biol Sci; FLOT1 promotes gastric cancer progression and metastasis through BCAR1/ERK signaling; Authors - Ran Wang ; KD Validated
rat	WB	Cell Death Dis; Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats; Authors - Yajie Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guorong (Verified Customer) (03-22-2022)

Good performance with a specific band of expected size and 2 bigger and weaker bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: ARPE-19

Flotillin 1 Antibody Western Blot validation (1:2500 dilution) in ARPE-19 (Cat no:15571-1-AP)