VEGFR3/FLT4 Polyklonaler Antikörper

VEGFR3/FLT4 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(17)

Rezensionen (1)

Kat-Nr. : 20712-1-AP

Synonyme

FLT4, Fms like tyrosine kinase 4, FLT41, FLT-4, FLT 4

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  WB analysis of mouse spleen using 20712-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 20712-1-AP (VEGFR3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20712-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20712-1-AP (VEGFR3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 20712-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20712-1-AP (VEGFR3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 20712-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20712-1-AP (VEGFR3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, HeLa-Zellen, Mausherzgewebe, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

20712-1-AP bindet in WB, IHC, IF, ELISA VEGFR3/FLT4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: fms-related tyrosine kinase 4
• Berechnetes Molekulargewicht: 153 kDa
• Beobachtetes Molekulargewicht: 125 kDa
• GenBank-Zugangsnummer: NM_002020
• Gene symbol: FLT4
• Gene ID (NCBI): 2324
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FLT4, also named VEGFR-3, belongs to the protein kinase superfamily, Tyr protein kinase family, and CSF-1/PDGF receptor subfamily. It is a receptor for VEGFC. FLT4 has a tyrosine-protein kinase activity. Defects in FLT4 cause lymphedema hereditary type 1 (LYH1A). Defects in FLT4 are found in juvenile hemangioma. VEGFR-3 is a highly glycosylated protein and migrates as bands with different molecular weights: an ~175 kDa precursor, an ~195 kDa mature form, an ~140 kDa non-glycosylated backbone, and a form that appears to be partially cleaved proteolytically in the extracellular domain to produce an ~125 kDa band(PMID: 15389531).

Publikationen

Species	Application	Title
human	IF	Mol Cancer; m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma; Authors - Han Fang
human	WB	Int J Biol Sci; PROTAC-Surufatinib Suppresses Pancreatic Neuroendocrine Neoplasms Progression by Inducing Ferroptosis through Inhibiting WNT/β-catenin Pathway Mediated by HMOX1; Authors - Bingyan Xue
human	IHC	Front Oncol; The Specifically Androgen-Regulated Gene (SARG) Promotes Papillary Thyroid Carcinoma (PTC) Lymphatic Metastasis Through Vascular Endothelial Growth Factor C (VEGF-C) and VEGF Receptor 3 (VEGFR-3) Axis.; Authors - Shuai-Jun Xu
Deer	WB	Stem Cell Res Ther; Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration.; Authors - Wei Zhang
human	WB	Pharmaceuticals (Basel); Use of Early Donated COVID-19 Convalescent Plasma Is Optimal to Preserve the Integrity of Lymphatic Endothelial Cells.; Authors - Nada Amri
human	WB	Mol Cell Endocrinol; Long noncoding RNA GAS5 accelerates diabetic wound healing and promotes lymphangiogenesis via miR-217/Prox1 axis.; Authors - Zhi-You He

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shinford (Verified Customer) (12-15-2023)

We used the concentration of 1:500 to get a very strong band. It is a big volume set.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Lung cancer cell lines, lung cancer tissues

VEGFR3/FLT4 Antibody Western Blot validation (1:500 dilution) in Lung cancer cell lines, lung cancer tissues (Cat no:20712-1-AP)