FMNL1 Polyklonaler Antikörper

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• KD/KO Validated

FMNL1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 27834-1-AP

Synonyme

CLL-associated antigen KW-13, FMNL, Formin-like protein 1

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  WB analysis using 27834-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27834-1-AP (FMNL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 27834-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27834-1-AP (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of Caki-1 using 27834-1-AP

  IP result of anti-FMNL1 (IP:27834-1-AP, 2ug; Detection:27834-1-AP 1:2000) with Caki-1 cells lysate 1000 ug. The result was provided by Keli Chai, Xuzhou Medical University.

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  IHC staining of human lymphoma using 27834-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27834-1-AP (FMNL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 27834-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27834-1-AP (FMNL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 27834-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 27834-1-AP (FMNL1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: Caki-1
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

27834-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA FMNL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FMNL1 fusion protein Ag27312
• Vollständiger Name: formin-like 1
• Beobachtetes Molekulargewicht: 125-150 kDa
• GenBank-Zugangsnummer: BC111564
• Gene symbol: FMNL1
• Gene ID (NCBI): 752
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. 27834-1-AP is a rabbit polyclonal antibody recognises the 125-150 kDa protein.(PMID:16494944)

Publikationen

Species	Application	Title
human	WB	Theranostics; Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer; Authors - Chen Li ; KD Validated
human	WB	Dev Cell; Vimentin intermediate filaments coordinate actin stress fibers and podosomes to determine the extracellular matrix degradation by macrophages; Authors - Xinyi Huang
human	IHC	Gastric Cancer; FHOD1 and FMNL1 formin proteins in intestinal gastric cancer: correlation with tumor-infiltrating T lymphocytes and molecular subtypes.; Authors - Naziha Mansuri
human	WB	Cell Rep; Substrate rigidity dictates colorectal tumorigenic cell stemness and metastasis via CRAD-dependent mechanotransduction.; Authors - Yuhan Chang
human	IHC	Biomed Res Int; Systematic Characterization of Expression Patterns and Immunocorrelations of Formin-Like Genes in Breast Cancer; Authors - Erli Gao
mouse	IHC	Clin Immunol; Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy; Authors - Jie Mei