FMNL1 Monoklonaler Antikörper

FMNL1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B12A4

Kat-Nr. : 67587-1-Ig

Synonyme

1B12A4, CLL-associated antigen KW-13, FMNL, Formin-like protein 1

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  WB analysis using 67587-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67587-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67587-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NK-92 using 67587-1-Ig

  NK-92 cells were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 67587-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using FMNL1 antibody (67587-1-Ig, Clone: 1B12A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HL-60-Zellen, K-562-Zellen, Hausschwein-Thymusgewebe
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67587-1-Ig bindet in WB, IF/ICC, ELISA FMNL1 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FMNL1 fusion protein Ag27322
• Vollständiger Name: formin-like 1
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC111564
• Gene symbol: FMNL1
• Gene ID (NCBI): 752
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. 67587-1-Ig antibody detects the protein around 140-150 kDa which is similar to the results reported in publications.(PMID: 21868368, 22986496 , 23182705)