FMO1 Rekombinanter Antikörper

FMO1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

230455B11

Kat-Nr. : 83156-3-RR

Synonyme

Trimethylamine monooxygenase, Flavin-containing monooxygenase 1, Fetal hepatic flavin-containing monooxygenase 1, EC:1.14.13.148, Dimethylaniline monooxygenase [N-oxide-forming] 1

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  WB analysis using 83156-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83156-3-RR (FMO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83156-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83156-3-RR against Human FMO1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe, Mauslebergewebe, Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83156-3-RR bindet in WB, ELISA FMO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FMO1 fusion protein Ag34267
• Vollständiger Name: flavin containing monooxygenase 1
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 53-60 kDa
• GenBank-Zugangsnummer: NM_001282692
• Gene symbol: FMO1
• Gene ID (NCBI): 2326
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Flavin-containing monooxygenase 1 (FMO1) belongs to the FMO family. FMO1 is an enzyme that catalyzes the oxygenation of a series of sulfur-containing and nitrogen-containing substrates, and thus is involved in a series of drugs and xenobiotics metabolism. FMO1 can regulate PPAR-α and mediate metabolic processes. FMO1 is expressed at relatively high levels in human fetal liver and also represents the major FMO enzyme in the adult human and rabbit intestine and kidney (PMID: 11809920, 30757917, 37553313). Human FMO1 has 2 isoforms with the molecular weights of 53 and 60 kDa. The observed molecular weight of FMO1 is 53 kDa, which might be the unmodified form of FMO1 (PMID: 9778310).