FMO5-specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FMO5-specific Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 16864-1-AP

Synonyme

FMO5, Baeyer-Villiger monooxygenase 1, Dimethylaniline monooxygenase [N-oxide-forming] 5, EC:1.14.13.-, EC:1.14.13.8

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  WB analysis using 16864-1-AP

  PC-3 cells and LNCap cells were subjected to SDS PAGE followed by western blot with 16864-1-AP (FMO5-specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 16864-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16864-1-AP (FMO5-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of multi-cells using 16864-1-AP

  WB result of 16864-1-AP from Dr.Soory.

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  IHC staining of human liver using 16864-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16864-1-AP (FMO5-specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16864-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16864-1-AP (FMO5-specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16864-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16864-1-AP (FMO5-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 16864-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16864-1-AP (FMO5-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse liver using 16864-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16864-1-AP (FMO5-specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16864-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16864-1-AP (FMO5-specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 16864-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 16864-1-AP (FMO5-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 16864-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug FMO5-specific Polyclonal antibody (16864-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, DU 145-Zellen, LNCaP-Zellen, MCF-7-Zellen, Mauslebergewebe, Rattenlebergewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Mammakarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

16864-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA FMO5-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: flavin containing monooxygenase 5
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: NM_001461
• Gene symbol: FMO5
• Gene ID (NCBI): 2330
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Microsomal flavin-containing monooxygenases (FMOs) [dimethylaniline monooxygenase (N-oxide forming) catalyze the FAD-, NADPH- and O2-dependent oxidation of a large number of structurally diverse compounds, including drugs, pesticides, and industrial chemicals containing a soft nucleophile(PMID:12488558 ). FMO5, which belongs to the FMO family, is a lesser component of human liver microsomes and is present at about one-third the level of FMO3. FMO5 protein is also present at very low levels in kidney, however, FMO5 exhibits a severely restricted substrate specificity for most drugs and other xenobiotics examined to date(PMID:10950857). It has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse	WB,IHC	Drug Metab Dispos; Identification of flavin-containing monooxygenase 5 (FMO5) as a regulator of glucose homeostasis and a potential sensor of gut bacteria.; Authors - Flora Scott ; KO Validated
mouse	IF	J Transl Med; Anthocyanin improves kidney function in diabetic kidney disease by regulating amino acid metabolism; Authors - Yi-Xi Li