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FMR1 Monoklonaler Antikörper
FMR1 Monoklonal Antikörper für WB, IHC, FC (Intra), IP, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, FC (Intra), IP, ELISA, IF
Konjugation
Unkonjugiert
CloneNo.
2B5B5
Kat-Nr. : 66548-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, HEK-293-Zellen, HSC-T6-Zellen, Jurkat-Zellen, K-562-Zellen |
| Erfolgreiche IP | HeLa-Zellen |
| Erfolgreiche Detektion in IHC | humanes Gliomgewebe, humanes Kolonkarzinomgewebe, humanes Kolongewebe, humanes Lebergewebe, Maushirngewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in FC (Intra) | Jurkat-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:250-1:1000 |
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IF | See 1 publications below |
Produktinformation
66548-1-Ig bindet in WB, IHC, FC (Intra), IP, ELISA, IF FMR1 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus |
| Wirt / Isotyp | Maus / IgG2a |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | FMR1 fusion protein Ag4697 |
| Vollständiger Name | fragile X mental retardation 1 |
| Berechnetes Molekulargewicht | 632 aa, 71 kDa |
| Beobachtetes Molekulargewicht | 69-72 kDa |
| GenBank-Zugangsnummer | BC038998 |
| Gene symbol | FMR1 |
| Gene ID (NCBI) | 2332 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for FMR1 antibody 66548-1-Ig | Protokoll herunterladen |
| IHC protocol for FMR1 antibody 66548-1-Ig | Protokoll herunterladenl |
| IP protocol for FMR1 antibody 66548-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Elife FMRP promotes RNA localization to neuronal projections through interactions between its RGG domain and G-quadruplex RNA sequences.
| ||
Environ Res Polychlorinated biphenyls alter hepatic m6A mRNA methylation in a mouse model of environmental liver disease | ||
Front Mol Neurosci Cingulate protein arginine methyltransferases 1 regulates peripheral hypersensitivity via fragile X messenger ribonucleoprotein |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Lisa (Verified Customer) (10-19-2021) | The antibody works quite well in WB, even if it is not possible to reuse the same diluted aliquot more than twice. I was not really satisfied of the results in IF, as the signal had a very high background.
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