FMR1NB Polyklonaler Antikörper

FMR1NB Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11069-2-AP

Synonyme

Cancer/testis antigen 37, CT37, FMR1NB, NY SAR 35, Sarcoma antigen NY SAR 35

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  WB analysis of MCF-7 using 11069-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11069-2-AP (FMR1NB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 11069-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11069-2-AP (FMR1NB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 11069-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11069-2-AP (FMR1NB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11069-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11069-2-AP (FMR1NB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11069-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11069-2-AP (FMR1NB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 11069-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11069-2-AP (FMR1NB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, A549-Zellen, MCF-7-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11069-2-AP bindet in WB, IHC, ELISA FMR1NB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FMR1NB fusion protein Ag1531
• Vollständiger Name: fragile X mental retardation 1 neighbor
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC034320
• Gene symbol: FMR1NB
• Gene ID (NCBI): 158521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.