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Fibronectin Polyklonaler Antikörper

Fibronectin Polyklonal Antikörper für FC, IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IP, IF, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15613-1-AP

Synonyme

CIG, Cold insoluble globulin, ED B, Fibronectin, fibronectin 1, FINC, FN, FN1, FNZ, GFND, GFND2, LETS, MSF



Geprüfte Anwendungen

Erfolgreiche Detektion in WBhuman plasma, MDA-MB-453s-Zellen, NIH/3T3-Zellen
Erfolgreiche IPNIH/3T3-Zellen
Erfolgreiche Detektion in IFNIH/3T3-Zellen
Erfolgreiche Detektion in FCNIH/3T3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:20000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15613-1-AP bindet in WB, IP, IF, FC, ELISA Fibronectin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, hamster, Hausschwein, Maus, Ratte, Medaka-Embryos
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Fibronectin fusion protein Ag8004
Vollständiger Name fibronectin 1
Berechnetes Molekulargewicht 2386 aa, 263 kDa
Beobachtetes Molekulargewicht 250-275 kDa
GenBank-ZugangsnummerBC005858
Gene symbol FN1
Gene ID (NCBI) 2335
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibronectins play a role in cell adhesion, motility, wound healing, and the maintenance of cell shape. Fibronectins bind to cell surfaces via interactions with integrins and various molecules such as collagen, fibrin, heparin, DNA, and actin (PMID: 3326130). Cellular interaction with fibronectin (FN1) promotes cell cycle progression and proliferation by influencing cyclins. 

What is the molecular weight of FN1?

The molecular weight of FN1 is 220 kDa. 

What is the tissue specificity of FN1?

FN1 is a large glycoprotein that exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1) (PMID: 21923916). Plasma FN1, or the dimeric form, is secreted by hepatocytes, whereas cellular FN1, the dimeric or cross-linked multimeric form, is produced by fibroblasts and epithelial cells and deposited as fibrils in the extracellular matrix. 

What are the post-translational modifications of FN1?

FN1 is often sulfated and its C-terminal NC1 peptide, anastellin, is produced as a result of proteolytic processing and can inhibit tumor growth, angiogenesis, and metastasis by activating p38 MAPK and inhibiting lysophospholipid signaling (PMID: 11209058).

What is FN1's role in embryogenesis?

Fibronectin has a crucial role in the development of the left-right body axis during embryogenesis (PMID: 21466802). 

What is FN1's involvement in disease?

Fibronectin glomerulopathy is a kidney disease that eventually leads to end-stage renal disease and is caused by mutations in the FN1 gene. Mutations in FN1 lead to the production of abnormal fibronectin protein that is deposited in the glomeruli of the kidney (PMID: 18268355).


Protokolle

Produktspezifische Protokolle
WB protocol for Fibronectin antibody 15613-1-APProtokoll herunterladen
IF protocol for Fibronectin antibody 15613-1-APProtokoll herunterladen
IP protocol for Fibronectin antibody 15613-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
rat

Adv Mater

Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine

Authors - Peng Lou
mouse

Cell Metab

The phytochemical hyperforin triggers thermogenesis in adipose tissue via a Dlat-AMPK signaling axis to curb obesity.

Authors - Suzhen Chen
mouseIF

J Clin Invest

Mitochondrial dysfunction in macrophages promotes inflammation and suppresses repair after myocardial infarction

Authors - Shanshan Cai
humanWB

Nat Protoc

Responsive and activable nanomedicines for remodeling the tumor microenvironment.

Authors - Yinlong Zhang
human

Nat Commun

Schwann cells regulate tumor cells and cancer-associated fibroblasts in the pancreatic ductal adenocarcinoma microenvironment

Authors - Meilin Xue
humanWB

Acta Pharm Sin B

KCTD4 interacts with CLIC1 to disrupt calcium homeostasis and promote metastasis in esophageal cancer

Authors - Cancan Zheng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ruchi (Verified Customer) (06-02-2023)

The Ab was tried on mouse skin on diabetic wounds.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Mouse skin
Fibronectin Antibody Immunofluorescence validation (1:50 dilution) in Mouse skin (Cat no:15613-1-AP)
FH

PK (Verified Customer) (03-20-2023)

excellent

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: fibroblast
Fibronectin Antibody Western Blot validation (1000 dilution) in fibroblast (Cat no:15613-1-AP)
FH

Susanne (Verified Customer) (08-23-2022)

under reduced conditions, blocked with 5% milk powder, incubation over night 4°C

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 : 1000
  • Cell Tissue Type: supernatant from human primary fibroblasts (serumfree !)
Fibronectin Antibody Western Blot validation (1 : 1000 dilution) in supernatant from human primary fibroblasts (serumfree !) (Cat no:15613-1-AP)
FH

Pankhuri (Verified Customer) (08-19-2022)

I tried this antibody for IF in neural stem cells. It works great in the conc. 1:200.

  • Applications: Immunofluorescence
  • Cell Tissue Type: Progenitor cells
Fibronectin Antibody Immunofluorescence validation ( dilution) in Progenitor cells (Cat no:15613-1-AP)
FH

Ren Jie (Verified Customer) (07-11-2022)

Very clean bands near 220 kD indicating fibronectin monomer

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human immortalized mammary fibroblasts
Fibronectin Antibody Western Blot validation (1:1000 dilution) in Human immortalized mammary fibroblasts (Cat no:15613-1-AP)
FH

SD (Verified Customer) (07-08-2022)

Good

  • Applications: Western Blot
  • Cell Tissue Type: Fibroblast
Fibronectin Antibody Western Blot validation ( dilution) in Fibroblast (Cat no:15613-1-AP)
FH

zunyi (Verified Customer) (03-11-2022)

This ab is used for immunohistochemistry on mouse paraffin-embedded sections. Antigen-retrieve, using pH 6.0 citric buffer, was performed prior to staining.

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: mouse bladder
FH

Lindsey (Verified Customer) (05-24-2021)

Works beautifully! Looking forward to working more with this antibody in different tissue/prep types

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human kidney (FFPE)
Fibronectin Antibody Immunohistochemistry,Immunofluorescence validation (1:100 dilution) in Human kidney (FFPE) (Cat no:15613-1-AP)