FN3K Polyklonaler Antikörper

FN3K Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 14293-1-AP

Synonyme

Protein-ribulosamine 3-kinase FN3K, Protein-psicosamine 3-kinase FN3K, Fructosamine-3-kinase, fructosamine 3 kinase, EC:2.7.1.172

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  WB analysis of HeLa using 14293-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14293-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14293-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14293-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 14293-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14293-1-AP

  IP result of anti-FN3K (IP:14293-1-AP, 3ug; Detection:14293-1-AP 1:500) with HeLa cells lysate 1840ug.

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  IHC staining of human kidney using 14293-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14293-1-AP (FN3K antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14293-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14293-1-AP (FN3K antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14293-1-AP

  Immunofluorescent analysis of HeLa cells using 14293-1-AP (FN3K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, humanes Herzgewebe, humanes Nierengewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

14293-1-AP bindet in WB, IF/ICC, IP, ELISA FN3K und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FN3K fusion protein Ag5617
• Vollständiger Name: fructosamine 3 kinase
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC042680
• Gene symbol: FN3K
• Gene ID (NCBI): 64122
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	iScience; Amiloride reduces fructosamine-3-kinase expression to restore sunitinib sensitivity in renal cell carcinoma; Authors - Yuanyuan Bai
human	WB	Cell Rep; A microscopy-based screen identifies cellular kinases modulating mitochondrial translation; Authors - Roya Yousefi