FNDC3B Polyklonaler Antikörper

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• KD/KO Validated

FNDC3B Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22605-1-PBS

Synonyme

FAD104, fibronectin type III domain containing 3B, Fibronectin type III domain-containing protein 3B, HCV NS5A-binding protein 37, UNQ2421/PRO4979/PRO34274

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  WB analysis of 4T1 using 22605-1-AP (same clone as 22605-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 22605-1-AP (FNDC3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22605-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 22605-1-AP (same clone as 22605-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 22605-1-AP (FNDC3B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22605-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 22605-1-AP (same clone as 22605-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 22605-1-AP (FNDC3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22605-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 22605-1-AP (same clone as 22605-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22605-1-AP (FNDC3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22605-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 22605-1-AP (same clone as 22605-1-PBS)

  IP result of anti-FNDC3B (IP:22605-1-AP, 4ug; Detection:22605-1-AP 1:1000) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 22605-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22605-1-AP (same clone as 22605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22605-1-AP (FNDC3B Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 22605-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22605-1-AP (same clone as 22605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22605-1-AP (FNDC3B Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 22605-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22605-1-PBS bindet in WB, IHC, IP, Indirect ELISA FNDC3B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FNDC3B fusion protein Ag18357
• Vollständiger Name: fibronectin type III domain containing 3B
• Berechnetes Molekulargewicht: 1204 aa, 133 kDa
• Beobachtetes Molekulargewicht: 150 kDa, 70 kDa
• GenBank-Zugangsnummer: BC039297
• Gene symbol: FNDC3B
• Gene ID (NCBI): 64778
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibronectin type III domain-containing protein 3B(FNDC3B), also named FAD104, belongs to the FNDC3 family. NDC3B is a positive regulator of adipocyte differentiation and is expressed at an early stage of adipocyte differentiation. FNDC3B is an endoplasmic reticulum transmembrane protein with a single transmembrane domain at the C terminus preceded by nine repeated fibronectin type III domains(PMID: 32966780). Due to the ability of the fibronectin type III domain to bind to a variety of proteins, FNDC3B plays a crucial role in cell adhesion, proliferation, and growth signaling. FNDC3B is abnormally expressed in a variety of human cancers, including hepatocellular carcinoma, acute myeloid leukemia, colorectal carcinoma, and cervical carcinoma(PMID: 36275754).