FNDC5 Polyklonaler Antikörper

FNDC5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(35)

Rezensionen (1)

Kat-Nr. : 23995-1-AP

Synonyme

Irisin, FRCP2, Fibronectin type III repeat-containing protein 2, Fibronectin type III domain-containing protein 5

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  WB analysis using 23995-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23995-1-AP (FNDC5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 23995-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23995-1-AP (FNDC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 23995-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23995-1-AP (FNDC5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 23995-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23995-1-AP (FNDC5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 23995-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23995-1-AP (FNDC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Maus-Skelettmuskelgewebe, Maus-Magengewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 28 publications below
• IHC: See 8 publications below
• IF: See 4 publications below

Produktinformation

23995-1-AP bindet in WB, IHC, IF, ELISA FNDC5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FNDC5 fusion protein Ag21195
• Vollständiger Name: fibronectin type III domain containing 5
• Berechnetes Molekulargewicht: 212 aa, 24 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC062297
• Gene symbol: FNDC5
• Gene ID (NCBI): 252995
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

fibronectin type III domain containing 5(FNDC5)encodes 23kDa protein, which is a membrane protein that is cleaved and secreted as a newly identified hormone, irisin. The exercise induces muscle FNDC5 expression. Exercise-induced FNDC5 gene expression in muscles was accompanied by a parallel increase in the concentration of circulating irisin, which in turn activates adipocyte thermogenic programs, leading to mitochondrial heat production and energy expenditure.

Publikationen

Species	Application	Title
	WB	Clin Transl Med; CRISPRa-based activation of Fgf21 and Fndc5 ameliorates obesity by promoting adipocytes browning; Authors - Hongtao Zhu
mouse	WB	Neuropathol Appl Neurobiol; Irisin treatment lowers levels of phosphorylated tau in the hippocampus of pre-symptomatic female but not male htau mice.; Authors - Katie A Bretland
mouse	WB	Br J Pharmacol; PMID: 31339170; Authors - Shengnan Shen
mouse	WB	Aging (Albany NY); Vitellogenin 2 promotes muscle development and stimulates the browning of white fat; Authors - Yilei Li
		J Am Heart Assoc; Irisin Lowers Blood Pressure by Improvement of Endothelial Dysfunction via AMPK-Akt-eNOS-NO Pathway in the Spontaneously Hypertensive Rat.; Authors - Jinjuan Fu
mouse	IHC	Biochim Biophys Acta Mol Basis Dis; Irisin is induced in renal ischemia-reperfusion to protect against tubular cell injury via suppressing p53.; Authors - Yuxue Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mohammad (Verified Customer) (12-02-2019)

1:600 in mice hippocampusworks well with no non-specific band

• Applications: Western Blot,