FNDC5 Rekombinanter Antikörper

FNDC5 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H3

Kat-Nr. : 82671-1-PBS

Synonyme

FNDC5, FRCP2

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  WB analysis using 82671-1-RR (same clone as 82671-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82671-1-RR (FNDC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82671-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 82671-1-RR (same clone as 82671-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82671-1-RR (FNDC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82671-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 82671-1-RR (same clone as 82671-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82671-1-RR (FNDC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82671-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82671-1-PBS bindet in WB, IHC, Indirect ELISA FNDC5 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FNDC5 fusion protein Ag29714
• Vollständiger Name: fibronectin type III domain containing 5
• Berechnetes Molekulargewicht: 212 aa, 24 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC062297
• Gene symbol: FNDC5
• Gene ID (NCBI): 252995
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

fibronectin type III domain containing 5(FNDC5) encodes 23 kDa protein, which is a membrane protein that is cleaved and secreted as a newly identified hormone, irisin. Exercise-induced FNDC5 gene expression in muscles was accompanied by a parallel increase in the concentration of circulating irisin, which in turn activates adipocyte thermogenic programs, leading to mitochondrial heat production and energy expenditure.