PSMA/GCPII Rekombinanter Antikörper

PSMA/GCPII Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242026F4

Kat-Nr. : 84615-2-PBS

Synonyme

FOLH1, PSMA, 242026F4, Cell growth-inhibiting gene 27 protein, EC:3.4.17.21

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  WB analysis of LNCaP using 84615-2-RR (same clone as 84615-2-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 84615-2-RR (FOLH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84615-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01454-1

  Sandwich ELISA standard curve of MP01454-1, Human PSMA/FOLH1 Recombinant Matched Antibody Pair - PBS only. 84615-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0216. 84615-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP01454-1

  Saliva of six individual healthy human donors was measured. The human PSMA/FOLH1 concentration of detected samples was determined to be 2.13 ng/mL with a range of 0.55 - 4.58 ng/mL.

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  Sample test of MP01454-1

  Serum of six individual healthy human donors was measured. The human PSMA/FOLH1 concentration of detected samples was determined to be 38.61 ng/mL with a range of 4.93 - 152.25 ng/mL.

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  Sample test of MP01454-1

  The mean PSMA/FOLH1 concentration was determined to be 2,098.65 ng/mL in LnCap cell extract based on a 2.0 mg/mL extract load.

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  IF Staining of LNCaP using 84615-2-RR (same clone as 84615-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using FOLH1 antibody (84615-2-RR, Clone: 242026F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84615-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01454-1

  Cytometric bead array standard curve of MP01454-1, PSMA/FOLH1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84615-2-PBS. Detection antibody: 84615-1-PBS. Standard: Eg0216. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84615-2-PBS

  Biolayer interferometry (BLl) kinetic assays of 84615-2-PBS against Human PSMA/GCPII were performed. The affinity constant is 3.46 nM.

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Geprüfte Anwendungen

Produktinformation

84615-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test PSMA/GCPII und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PSMA/GCPII fusion protein Eg0216
• Vollständiger Name: folate hydrolase (prostate-specific membrane antigen) 1
• Berechnetes Molekulargewicht: 84KD
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: NM_004476.3
• Gene symbol: PSMA
• Gene ID (NCBI): 2346
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Prostate-specific membrane antigen (PSMA), also known as glutamate carboxypeptidase II (GCPII), is a type II transmembrane glycoprotein that has gained significant attention in the field of prostate cancer (PCa) diagnostics and therapeutics. PSMA is highly overexpressed in prostate cancer tissue, especially in aggressive, androgen-deprived, metastatic, and hormone-refractory prostate cancers. Up to 95% of prostate adenocarcinomas express PSMA receptors on their surface. Physiological expression of PSMA is observed in normal prostate epithelium, duodenum, colon, renal tubules, and sympathetic ganglia. It is also found in other tissues such as the salivary glands, lacrimal glands, and various parts of the gastrointestinal tract, though at lower levels.