PSMA/GCPII Rekombinanter Antikörper

PSMA/GCPII Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242026F4

Kat-Nr. : 84615-2-RR

Synonyme

FOLH1, PSMA, 242026F4, Cell growth-inhibiting gene 27 protein, EC:3.4.17.21

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  WB analysis of LNCaP using 84615-2-RR

  LNCaP cells were subjected to SDS PAGE followed by western blot with 84615-2-RR (FOLH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of LNCaP using 84615-2-RR

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using FOLH1 antibody (84615-2-RR, Clone: 242026F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen
• Erfolgreiche Detektion in IF/ICC: LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84615-2-RR bindet in WB, IF/ICC, ELISA PSMA/GCPII und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PSMA/GCPII fusion protein Eg0216
• Vollständiger Name: folate hydrolase (prostate-specific membrane antigen) 1
• Berechnetes Molekulargewicht: 84KD
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: NM_004476.3
• Gene symbol: PSMA
• Gene ID (NCBI): 2346
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Prostate-specific membrane antigen (PSMA), also known as glutamate carboxypeptidase II (GCPII), is a type II transmembrane glycoprotein that has gained significant attention in the field of prostate cancer (PCa) diagnostics and therapeutics. PSMA is highly overexpressed in prostate cancer tissue, especially in aggressive, androgen-deprived, metastatic, and hormone-refractory prostate cancers. Up to 95% of prostate adenocarcinomas express PSMA receptors on their surface. Physiological expression of PSMA is observed in normal prostate epithelium, duodenum, colon, renal tubules, and sympathetic ganglia. It is also found in other tissues such as the salivary glands, lacrimal glands, and various parts of the gastrointestinal tract, though at lower levels.