FOLR1 Polyklonaler Antikörper

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FOLR1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23355-1-PBS

Synonyme

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  WB analysis of HeLa using 23355-1-AP (same clone as 23355-1-PBS)

  WB result of FOLR1 antibody (23355-1-AP, 1:1000) with si-Control and si-FOLR1 transfected HeLa cells.. This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

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  WB analysis using 23355-1-AP (same clone as 23355-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23355-1-AP (FOLR1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 23355-1-AP (same clone as 23355-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23355-1-AP (FOLR1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 23355-1-AP (same clone as 23355-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23355-1-AP (FOLR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 23355-1-AP (same clone as 23355-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23355-1-AP (FOLR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 23355-1-AP (same clone as 23355-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FOLR1 antibody (23355-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

23355-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA FOLR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FOLR1 fusion protein Ag19959
• Vollständiger Name: folate receptor 1 (adult)
• Berechnetes Molekulargewicht: 257 aa, 30 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC002947
• Gene symbol: FOLR1
• Gene ID (NCBI): 2348
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Folate receptor 1 (FOLR1), also known as folate receptor alpha or adult folate-binding protein (FBP), is a 38-kDa glycoprotein belonging to the folate receptor family (PMID:15094207; 23528302). The receptor binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. FOLR1 expression is often limited to the apical surfaces of epithelium in the lung, kidney and choroid plexus but is differentially overexpressed in a variety of solid tumors such as ovarian cancer, non-small cell lung cancer, breast cancer, kidney cancer and high-grade osteosarcoma (PMID: 23528302; 23357463).