FOLR1 Monoklonaler Antikörper

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• KD/KO Validated

FOLR1 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

2B4B7

Kat-Nr. : 60307-1-Ig

Synonyme

2B4B7, Adult folate binding protein, Adult folate-binding protein, FBP, Folate receptor 1

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  WB analysis of HeLa using 60307-1-Ig

  WB result of FOLR1 antibody (60307-1-Ig, 1:4000) with si-Control and si-FOLR1 transfected HeLa cells..

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  WB analysis of human milk using 60307-1-Ig

  human milk were subjected to SDS PAGE followed by western blot with 60307-1-Ig (FOLR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60307-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60307-1-Ig (FOLR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 60307-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60307-1-Ig (FOLR1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 60307-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60307-1-Ig (FOLR1 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human milk, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

60307-1-Ig bindet in WB, IHC, ELISA FOLR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FOLR1 fusion protein Ag19959
• Vollständiger Name: folate receptor 1 (adult)
• Berechnetes Molekulargewicht: 257 aa, 30 kDa
• Beobachtetes Molekulargewicht: 36-38 kDa
• GenBank-Zugangsnummer: BC002947
• Gene symbol: FOLR1
• Gene ID (NCBI): 2348
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Folate receptor 1 (FOLR1), also known as folate receptor alpha or adult folate-binding protein (FBP), is a 38-kDa glycoprotein belonging to the folate receptor family (PMID:15094207; 23528302). The receptor binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. FOLR1 expression is often limited to the apical surfaces of epithelium in the lung, kidney and choroid plexus but is differentially overexpressed in a variety of solid tumors such as ovarian cancer, non-small cell lung cancer, breast cancer, kidney cancer and high-grade osteosarcoma (PMID: 23528302; 23357463).

Publikationen

Species	Application	Title
human	WB	ACS Nano; High-Performance Self-Cascade Pyrite Nanozymes for Apoptosis-Ferroptosis Synergistic Tumor Therapy.; Authors - Xiangqin Meng
human	WB	Biosensors (Basel); Folic Acid-Modified Fluorescent-Magnetic Nanoparticles for Efficient Isolation and Identification of Circulating Tumor Cells in Ovarian Cancer.; Authors - Yue Pan
human	WB	Antibodies (Basel); Novel Anti-FOLR1 Antibody-Drug Conjugate MORAb-202 in Breast Cancer and Non-Small Cell Lung Cancer Cells.; Authors - Yuki Matsunaga ; KD Validated
		J Mol Diagn; Colocalization of cancer-associated biomarkers on single extracellular vesicles for early detection of cancer; Authors - Daniel P Salem
human	IHC	Arch Pathol Lab Med; Evaluation of Laboratory-Derived Immunohistochemical Assays for Folate Receptor α Expression in Epithelial Ovarian Cancer and Comparison With a Companion Diagnostic; Authors - Emily Deutschman
human	IHC	Cells; Mirvetuximab Soravtansine Induces Potent Cytotoxicity and Bystander Effect in Cisplatin-Resistant Germ Cell Tumor Cells; Authors - Lucia Kucerova