FOX2/RBM9 Polyklonaler Antikörper

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• KD/KO Validated

FOX2/RBM9 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12498-1-PBS

Synonyme

FOX2, RBM9, Fox 2, Fox-1 homolog B, fxh

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  WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

  WB result of FOX2/RBM9 antibody (12498-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOX2/RBM9 transfected HEK-293 cells. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 12498-1-AP (same clone as 12498-1-PBS)

  IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with HepG2 cells lysate 2680 ug. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 12498-1-AP (same clone as 12498-1-PBS)

  IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with K-562 cells lysate 1760 ug. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 12498-1-AP (same clone as 12498-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12498-1-AP (same clone as 12498-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOX2/RBM9 antibody (12498-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12498-1-PBS bindet in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA FOX2/RBM9 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FOX2/RBM9 fusion protein Ag3168
• Vollständiger Name: RNA binding motif protein 9
• Berechnetes Molekulargewicht: 390 aa, 41 kDa
• Beobachtetes Molekulargewicht: 50- 60 kDa
• GenBank-Zugangsnummer: BC013115
• Gene symbol: RBM9
• Gene ID (NCBI): 23543
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)