FOXA1 Polyklonaler Antikörper

FOXA1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 20411-1-AP

Synonyme

forkhead box A1, Forkhead box protein A1, FOXA1, HNF 3 alpha, HNF 3A, HNF3A, TCF 3A, TCF3A, Transcription factor 3A

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Galerie der Validierungsdaten

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  WB analysis using 20411-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20411-1-AP (FOXA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20411-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20411-1-AP (FOXA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human urothelial carcinoma using 20411-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 20411-1-AP (FOXA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 20411-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 20411-1-AP (FOXA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HepG2-Zellen, MDA-MB-468-Zellen und T-47D-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• CoIP: See 1 publications below

Produktinformation

20411-1-AP bindet in WB, CoIP, ELISA FOXA1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FOXA1 fusion protein Ag14243
• Vollständiger Name: forkhead box A1
• Berechnetes Molekulargewicht: 473 aa, 49 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC033890
• Gene symbol: FOXA1
• Gene ID (NCBI): 3169
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Forkhead box A1(FOXA1),also named hepatocyte nuclear factor 3-alpha (HNF-3A), is a transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.

Publikationen

Species	Application	Title
human	WB	Cancer Lett; An SGLT2 inhibitor modulates SHH expression by activating AMPK to inhibit the migration and induce the apoptosis of cervical carcinoma cells.; Authors - Zipeng Xie
human	WB	J Pathol; Down-regulation of TRPS1 stimulates epithelial-mesenchymal transition and metastasis through repression of FOXA1.; Authors - Jin-Zhou Huang
human	WB	J Exp Clin Cancer Res; A novel FBW7/NFAT1 axis regulates cancer immunity in sunitinib-resistant renal cancer by inducing PD-L1 expression.; Authors - Wentao Liu
human	WB	Oncotarget; miR-194 inhibits the proliferation, invasion, migration, and enhances the chemosensitivity of non-small cell lung cancer cells by targeting forkhead box A1 protein.; Authors - Xuchao Zhu
human	WB	Oncol Lett; Upregulated forkhead-box A3 elevates the expression of forkhead-box A1 and forkhead-box A2 to promote metastasis in esophageal cancer.; Authors - Bing Chen
human,mouse	WB	Stem Cell Res Ther; Knockdown of FOXA1 enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells partly via activation of the ERK1/2 signalling pathway; Authors - Lijun Li ; KD Validated