FOXA2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FOXA2 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Rezensionen (2)

Kat-Nr. : 22474-1-AP

Synonyme

forkhead box A2, Forkhead box protein A2, FOXA2, HNF 3 beta, HNF 3B, HNF3B, TCF 3B, TCF3B, Transcription factor 3B

---

Galerie der Validierungsdaten

• 

  WB analysis using 22474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22474-1-AP (FOXA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 22474-1-AP

  IP Result of anti-FOXA2 (IP:22474-1-AP, 3ug; Detection:22474-1-AP 1:300) with HepG2 cells lysate 1920ug.

• 

  IHC staining of human pancreas using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22474-1-AP (FOXA2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22474-1-AP (FOXA2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22474-1-AP (FOXA2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22474-1-AP (FOXA2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22474-1-AP (FOXA2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22474-1-AP (FOXA2 Antibody) at dilution of 1:50 (under 10x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, A549-Zellen, HepG2-Zellen, MDA-MB-231-Zellen
• Erfolgreiche IP: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• ChIP: See 1 publications below

Produktinformation

22474-1-AP bindet in WB, IP, IHC, IF, chIP, ELISA FOXA2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FOXA2 fusion protein Ag18221
• Vollständiger Name: forkhead box A2
• Berechnetes Molekulargewicht: 457 aa, 48 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: BC011780
• Gene symbol: FOXA2
• Gene ID (NCBI): 3170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXA2 also named as HNF 3 beta or TCF3B is a 457 amino acid protein, which contains one fork-head DNA-binding domains. FOXA2 can Shuttle between the nucleus and cytoplasm in a CRM1-dependent manner. Phosphorylation on FOXA2 Thr-156 abolishes binding to target promoters and subsequent transcription activation upon INS stimulation. FOXA2 as a transcription factor involves in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. The molecular weight of FOXA2 is 48kDa, but the phosphorylated FOXA2 may be a 55-65kDa protein.

Publikationen

Species	Application	Title
human	chIP	Cell Res; Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer.; Authors - Shuaishuai Teng
human	IF	Chemosphere; Comparative cytotoxicity studies of halophenolic disinfection byproducts using human extended pluripotent stem cells.; Authors - Yujie Liu
human	WB,IHC,IF	Am J Cancer Res; Epigenetically silenced linc00261 contributes to the metastasis of hepatocellular carcinoma via inducing the deficiency of FOXA2 transcription.; Authors - Zhanjun Chen ; KD Validated
human	WB	Int J Biol Sci; EGF suppresses the expression of miR-124a in pancreatic β cell lines via ETS2 activation through the MEK and PI3K signaling pathways.; Authors - Lin Yang
mouse	WB	Mol Cell Endocrinol; Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.; Authors - Xinghong Guo
human	IF	Ann Transl Med; Changes in the hepatic differentiation potential of human mesenchymal stem cells aged in vitro.; Authors - Sang Luo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Kamal (Verified Customer) (07-05-2023)

Worked when diluted in TBST for WB and 1X PBS for IFC.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 (WB) and 1:200 (IFC)
• Cell Tissue Type: Mouse Liver tissue

FH

Kamal (Verified Customer) (07-05-2023)

Worked when diluted in TBST for WB and 1X PBS for IFC.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 (WB) and 1:200 (IFC)
• Cell Tissue Type: Mouse Liver tissue