FOXA2 Polyklonaler Antikörper

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• KD/KO Validated

FOXA2 Polyklonal Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22474-1-PBS

Synonyme

forkhead box A2, Forkhead box protein A2, Hepatocyte nuclear factor 3-beta, HNF 3 beta, HNF 3B

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  WB analysis using 22474-1-AP (same clone as 22474-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22474-1-AP (FOXA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22474-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 22474-1-AP (same clone as 22474-1-PBS)

  WB result of FOXA2 antibody (22474-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and sg-FOXA2 transfected HepG2 cells. This data was developed using the same antibody clone with 22474-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 22474-1-AP (same clone as 22474-1-PBS)

  IP result of anti-FOXA2 (IP:22474-1-AP, 4ug; Detection:22474-1-AP 1:500) with HepG2 cells lysate 1320 ug. This data was developed using the same antibody clone with 22474-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 22474-1-AP (same clone as 22474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22474-1-AP (FOXA2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22474-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 22474-1-AP (same clone as 22474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22474-1-AP (FOXA2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22474-1-PBS in a different storage buffer formulation.

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  IF Staining of rat stomach using 22474-1-AP (same clone as 22474-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat stomach tissue using FOXA2 antibody (22474-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22474-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22474-1-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA FOXA2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FOXA2 fusion protein Ag18221
• Vollständiger Name: forkhead box A2
• Berechnetes Molekulargewicht: 457 aa, 48 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: BC011780
• Gene symbol: FOXA2
• Gene ID (NCBI): 3170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXA2 also named as HNF 3 beta or TCF3B is a 457 amino acid protein, which contains one fork-head DNA-binding domains. FOXA2 can Shuttle between the nucleus and cytoplasm in a CRM1-dependent manner. Phosphorylation on FOXA2 Thr-156 abolishes binding to target promoters and subsequent transcription activation upon INS stimulation. FOXA2 as a transcription factor involves in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. The molecular weight of FOXA2 is 48kDa, but the phosphorylated FOXA2 may be a 55-65kDa protein.