FOXC2 Rekombinanter Antikörper

FOXC2 Rekombinant Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240403H7

Kat-Nr. : CL488-83476-5

Synonyme

240403H7, FKHL14, Forkhead box protein C2, Forkhead-related protein FKHL14, Mesenchyme fork head protein 1

---

• 

  IF Staining of A375 using CL488-83476-5

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using CoraLite® Plus 488 FOXC2 antibody (CL488-83476-5, Clone: 240403H7 ) at dilution of 1:200, CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-83476-5 bindet in IF/ICC FOXC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FOXC2 fusion protein Ag33121
• Vollständiger Name: forkhead box C2 (MFH-1, mesenchyme forkhead 1)
• Berechnetes Molekulargewicht: 54kd
• GenBank-Zugangsnummer: NM_005251
• Gene symbol: FOXC2
• Gene ID (NCBI): 2303
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Forkhead box protein C2 (FOXC2) also known as forkhead-related protein FKHL14 (FKHL14), transcription factor FKH-14, or mesenchyme fork head protein 1 (MFH1) is a protein that in humans is encoded by the FOXC2 gene. FOXC2 is a member of the fork head box (FOX) family of transcription factors. FOX transcription factors are expressed during development and are associated with a number of cellular and developmental differentiation processes. FOXC2 is required during early development of the kidneys, including differentiation of podocytes and maturation of the glomerular basement membrane. It is also involved in the early development of the heart. FOXC2 is also involved in cancer metastases. In particular, expression of FOXC2 is induced when epithelial cells undergo an epithelial-mesenchymal transition (EMT) and become mesenchymal looking cells. (PMID: 8674414 9169153 19935708) This antibody recognizes the 56 kDa FOXC2 protein, but the phosphorylated FOXC2 may be a 56-65 kDa protein. The 62kDa band was also detected in the study(PMID: 19540201).