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FOXF1 Rekombinanter Antikörper

FOXF1 Rekombinant Antikörper für IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241076C8

Kat-Nr. : 84020-3-PBS

Synonyme

241076C8

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FOXF1 Recombinant antibody (84020-3-RR)

    IF Staining of HeLa using 84020-3-RR (same clone as 84020-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FOXF1 antibody (84020-3-RR, Clone: 241076C8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84020-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FOXF1 Recombinant antibody (84020-3-RR)

    IF Staining of HeLa using 84020-3-RR (same clone as 84020-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FOXF1 antibody (84020-3-RR, Clone: 241076C8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84020-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using FOXF1 Recombinant antibody (84020-3-RR)

    FC experiment of HeLa using 84020-3-RR (same clone as 84020-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug FOXF1 Recombinant antibody (84020-3-RR, Clone:241076C8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84020-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84020-3-RR

    Affinity and Kinetic Characterization of 84020-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84020-3-RR against Human FOXF1 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

84020-3-PBS bindet in IF/ICC, FC (Intra), Indirect ELISA FOXF1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen FOXF1 fusion protein Ag34266
Vollständiger Name forkhead box F1
Berechnetes Molekulargewicht40 kDa
Beobachtetes Molekulargewicht40 kDa
GenBank-ZugangsnummerNP_001442
Gene symbol FOXF1
Gene ID (NCBI) 2294
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXF1 (Forkhead box protein F1), also known as FKHL5, It is expected to be located in the nucleus, which is expressed predominantly in lung and placenta. FOXF1, a member of the Forkhead box family of transcription factors, as one of the most highly and consistently upregulated genes in FP-RMS. While it is known that FOXF1 is required for mesodermal differentiation toward endothelial and smooth muscle cell lineages, FOXF1 is not expressed in differentiated skeletal muscle cells, and its role in fusion-positive FP-RMS remains unknown (PMID: 33627785). The molecular weight of FOXF1 is 40 kDa.