FOXK1 Polyklonaler Antikörper

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• KD/KO Validated

FOXK1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29338-1-PBS

Synonyme

FOXK1L, MNF

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  WB analysis using 29338-1-AP (same clone as 29338-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29338-1-AP (FOXK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29338-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 29338-1-AP (same clone as 29338-1-PBS)

  WB result of FOXK1 antibody (29338-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXK1 transfected Jurkat cells. This data was developed using the same antibody clone with 29338-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 29338-1-AP (same clone as 29338-1-PBS)

  IP result of anti-FOXK1 (IP:29338-1-AP, 4ug; Detection:29338-1-AP 1:3000) with K-562 cells lysate 1800 ug. This data was developed using the same antibody clone with 29338-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29338-1-AP (same clone as 29338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29338-1-AP (FOXK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29338-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29338-1-AP (same clone as 29338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29338-1-AP (FOXK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29338-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SW480 using 29338-1-AP (same clone as 29338-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SW480 cells using FOXK1 antibody (29338-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 29338-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29338-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA FOXK1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FOXK1 fusion protein Ag28820
• Vollständiger Name: forkhead box K1
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: NM_001037165
• Gene symbol: FOXK1
• Gene ID (NCBI): 221937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Forkhead box (FOX) domains bind DNA and consist of 2 loops connecting beta-sheets that flank an alpha-helix, which is a group of highly conserved transcription factors. Forkhead box k1 (FOXK1) also known as MNF, is a transcription factor that belongs to the forkhead family consisting of the winged-helix DNA-binding domain and the N-terminal and C-terminal transcriptional domains(PMID: 15289879, PMID: 27223064). There are two isoforms of FoxK1: FoxK1-α and FoxK1-β. FoxK1-α (90 kDa) is expressed in committed myoblasts and differentiated myotubes, while FoxK1-β (55 kDa) is expressed mainly in quiescent satellite cells(PMID: 9271401).