FOXK1 Rekombinanter Antikörper

FOXK1 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251186A11

Kat-Nr. : 86452-1-PBS

Synonyme

FOXK1L, MNF

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  WB analysis using 86452-1-RR (same clone as 86452-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86452-1-RR (FOXK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86452-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 86452-1-RR (same clone as 86452-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86452-1-RR (FOXK1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86452-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 86452-1-RR (same clone as 86452-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOXK1 antibody (86452-1-RR, Clone: 251186A11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86452-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

86452-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA FOXK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FOXK1 fusion protein Ag28820
• Vollständiger Name: forkhead box K1
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: NM_001037165
• Gene symbol: FOXK1
• Gene ID (NCBI): 221937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Forkhead box (FOX) domains bind DNA and consist of 2 loops connecting beta-sheets that flank an alpha-helix, which is a group of highly conserved transcription factors. Forkhead box k1 (FOXK1) also known as MNF, is a transcription factor that belongs to the forkhead family consisting of the winged-helix DNA-binding domain and the N-terminal and C-terminal transcriptional domains(PMID: 15289879, PMID: 27223064). There are two isoforms of FoxK1: FoxK1-α and FoxK1-β. FoxK1-α (90 kDa) is expressed in committed myoblasts and differentiated myotubes, while FoxK1-β (55 kDa) is expressed mainly in quiescent satellite cells(PMID: 9271401).