FOXK2 Polyklonaler Antikörper

FOXK2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30660-1-AP

Synonyme

forkhead box K2, Forkhead box protein K2, FOXK1, FOXK2, ILF, ILF 1, ILF1

---

Galerie der Validierungsdaten

• 

  WB analysis using 30660-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30660-1-AP (FOXK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30660-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30660-1-AP (FOXK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 30660-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30660-1-AP (FOXK2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30660-1-AP bindet in WB, IHC, ELISA FOXK2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FOXK2 fusion protein Ag33625
• Vollständiger Name: forkhead box K2
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 69-79 kDa
• GenBank-Zugangsnummer: NM_004514.3
• Gene symbol: FOXK2
• Gene ID (NCBI): 3607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXK2 (forkhead box K2), also known as ILF and ILF1. FOXK2 is localized at nucleus and cytoplasm. It is also expected to be expressed in testis, brain and lymphoid and non-lymphoid cells (PMID: 1339390). The protein encoded by this gene contains a fork head DNA binding domain. This protein can bind to the purine-rich motifs of the HIV long terminal repeat (LTR), and to the similar purine-rich motif in the interleukin 2 (IL2) promoter. It may be involved in the regulation of viral and cellular promoter elements. And its molecular weight is 69 kDa. Anett Marais demonstrate that FOXK2 is phosphorylated in a cell cycle-dependent manner. This phosphorylation peaks during M phase and is mediated by CDK·cyclin complexes. FOXK2 exhibits a periodic rise in its phosphorylation levels during the cell cycle, with hyperphosphorylation occurring in mitotic cells (PMID: 20810654).