FOXK2 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

FOXK2 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240692H4

Kat-Nr. : 83735-6-RR

Synonyme

240692H4, forkhead box K2, Forkhead box protein K2, ILF, ILF 1

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  WB analysis using 83735-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83735-6-RR (FOXK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 83735-6-RR

  WB result of FOXK2 antibody (83735-6-RR; 1:10000; room temperature for 1.5 hours) with wild-type and sg-FOXK2 transfected HAP1 cells.

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  IHC staining of human placenta using 83735-6-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83735-6-RR (FOXK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 83735-6-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83735-6-RR (FOXK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 83735-6-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83735-6-RR (FOXK2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 83735-6-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83735-6-RR (FOXK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 83735-6-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83735-6-RR (FOXK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 83735-6-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FOXK2 antibody (83735-6-RR, Clone: 240692H4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of SH-SY5Y using 83735-6-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Foxk2 Recombinant Antibody (83735-6-RR, Clone:240692H4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HEK-293T using 83735-6-RR

  1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Foxk2 Recombinant Antibody (83735-6-RR, Clone:240692H4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83735-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83735-6-RR against Human FOXK2 were performed. The affinity constant is 0.347 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, MCF-7-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Kolongewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293T-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83735-6-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA FOXK2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FOXK2 fusion protein Ag33625
• Vollständiger Name: forkhead box K2
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 69-79 kDa
• GenBank-Zugangsnummer: NM_004514.3
• Gene symbol: FOXK2
• Gene ID (NCBI): 3607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXK2 (forkhead box K2), also known as ILF and ILF1. FOXK2 is localized at nucleus and cytoplasm. It is also expected to be expressed in testis, brain and lymphoid and non-lymphoid cells (PMID: 1339390). The protein encoded by this gene contains a fork head DNA binding domain. This protein can bind to the purine-rich motifs of the HIV long terminal repeat (LTR), and to the similar purine-rich motif in the interleukin 2 (IL2) promoter. It may be involved in the regulation of viral and cellular promoter elements. And its molecular weight is 69 kDa. Anett Marais demonstrate that FOXK2 is phosphorylated in a cell cycle-dependent manner. This phosphorylation peaks during M phase and is mediated by CDK·cyclin complexes. FOXK2 exhibits a periodic rise in its phosphorylation levels during the cell cycle, with hyperphosphorylation occurring in mitotic cells (PMID: 20810654).