FOXM1 Rekombinanter Antikörper

FOXM1 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242077A7

Kat-Nr. : 84681-1-RR

Synonyme

HNF-3/fork-head homolog 11, HFH-11, HFH11, Forkhead-related protein FKHL16, FKHL16

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  WB analysis using 84681-1-RR

  A431 cells were subjected to SDS PAGE followed by western blot with 84681-1-RR (FOXM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84681-1-RR

  L02 cells were subjected to SDS PAGE followed by western blot with 84681-1-RR (FOXM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 84681-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug FOXM1 Recombinant antibody (84681-1-RR, Clone:242077A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  FC experiment of A549 using 84681-1-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug FOXM1 Recombinant antibody (84681-1-RR, Clone:242077A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84681-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84681-1-RR against Human FOXM1 were performed. The affinity constant is 10.7 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, A431-Zellen, HT-29-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84681-1-RR bindet in WB, FC (Intra), ELISA FOXM1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FOXM1 fusion protein Ag3729
• Vollständiger Name: forkhead box M 1
• Berechnetes Molekulargewicht: 83 kDa
• Beobachtetes Molekulargewicht: 84-110 kDa
• GenBank-Zugangsnummer: BC006192
• Gene symbol: FOXM1
• Gene ID (NCBI): 2305
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Forkhead box M1 (FOXM1), also named as HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly as5sociated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is a rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1, FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa). Sometimes a molecular weight of about 65 kDa can also be observed, which may be a degradation form of FOXM1 (PMID: 26547933).