FOXO1 Monoklonaler Antikörper

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• KD/KO Validated

FOXO1 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

CloneNo.

1D9A2

Rezensionen (1)

Kat-Nr. : 66457-1-Ig

Synonyme

1D9A2, FKH 1, FKH1, FKHR, forkhead box O1

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• 

  WB analysis using 66457-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66457-1-Ig

  WB result of FOXO1 antibody (66457-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXO1 transfected HEK-293 cells.

• 

  WB analysis of HeLa using 66457-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 66457-1-Ig

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 66457-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66457-1-Ig (FOXO1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66457-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66457-1-Ig (FOXO1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, L02-Zellen, SMMC-7721-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

66457-1-Ig bindet in WB, IHC, IF, CoIP, ELISA FOXO1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ziege
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FOXO1 fusion protein Ag13296
• Vollständiger Name: forkhead box O1
• Berechnetes Molekulargewicht: 655 aa, 70 kDa
• Beobachtetes Molekulargewicht: 68-70 kDa
• GenBank-Zugangsnummer: BC021981
• Gene symbol: FOXO1
• Gene ID (NCBI): 2308
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).

Publikationen

Species	Application	Title
human	WB	Transl Psychiatry; Psychosocial and behavioral problems of children and adolescents in the early stage of reopening schools after the COVID-19 pandemic: a national cross-sectional study in China.; Authors - Lin Wang
human	WB	Int J Biol Sci; ATIC inhibits autophagy in hepatocellular cancer through the AKT/FOXO3 pathway and serves as a prognostic signature for modeling patient survival.; Authors - Hao Zhang
human	WB	Int Immunopharmacol; 1β-Hydroxyalantolactone from Inulae Flos alleviated the progression of pulmonary fibrosis via inhibiting JNK/FOXO1/NF-κB pathway; Authors - Bing Yu
human	WB	Thorac Cancer; Serum exosomal miR-1269a serves as a diagnostic marker and plays an oncogenic role in non-small cell lung cancer.; Authors - Xue Wang ; KD Validated
	WB	Equine Vet J; Muscle fibre transition and transcriptional changes of horse skeletal muscles during traditional Mongolian endurance training; Authors - Tugeqin Bou
mouse	WB	Ecotoxicol Environ Saf; Integrated network toxicology, molecular docking, and in vivo experiments to elucidate molecular mechanism of aflatoxin B1 hepatotoxicity; Authors - Bingjie Ge

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Refik (Verified Customer) (09-07-2025)

Works well with overnight incubation with 1:250 in 1x PBST with 5% BSA for ICC staining of human-derived cells with 3 hours at RTP with 1:400 secondary antibody in 1x PBST with 5% Donkey Serum and DAPI

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:250 for ICC staining of human-derived cells with 1:400 secondary antibody
• Cell Tissue Type: Human-derived cardiac cells