FOXO1 Monoklonaler Antikörper

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• KD/KO Validated

FOXO1 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D9A2

Kat-Nr. : 66457-1-PBS

Synonyme

1D9A2, FKH 1, FKH1, FKHR, forkhead box O1

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  WB analysis using 66457-1-Ig (same clone as 66457-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66457-1-Ig (same clone as 66457-1-PBS)

  WB result of FOXO1 antibody (66457-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXO1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66457-1-Ig (same clone as 66457-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SMMC-7721 using 66457-1-Ig (same clone as 66457-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66457-1-Ig (same clone as 66457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66457-1-Ig (FOXO1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66457-1-Ig (same clone as 66457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66457-1-Ig (FOXO1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66457-1-PBS bindet in WB, IHC, ELISA FOXO1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FOXO1 fusion protein Ag13296
• Vollständiger Name: forkhead box O1
• Berechnetes Molekulargewicht: 655 aa, 70 kDa
• Beobachtetes Molekulargewicht: 68-70 kDa
• GenBank-Zugangsnummer: BC021981
• Gene symbol: FOXO1
• Gene ID (NCBI): 2308
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).