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  • KD/KO Validated

FOXO3A Monoklonaler Antikörper

FOXO3A Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(58)

CloneNo.

1F12D11

Kat-Nr. : 66428-1-Ig

Synonyme

AF6q21, AF6q21 protein, FKHRL1, FKHRL1P2, forkhead box O3, Forkhead box protein O3, FOXO2, FOXO3, FOXO3A



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen, PC-12-Zellen
Erfolgreiche IPHEK-293-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Ovarialkarzinomgewebe, humanes Magenkrebsgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:250-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66428-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA FOXO3A und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte, Zebrafisch, Makake
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen FOXO3A fusion protein Ag1289
Vollständiger Name forkhead box O3
Berechnetes Molekulargewicht 673 aa, 71 kDa
Beobachtetes Molekulargewicht 85-90 kDa
GenBank-ZugangsnummerBC021224
Gene symbol FOXO3A
Gene ID (NCBI) 2309
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa,but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa fulllength FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after INS stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for FOXO3A antibody 66428-1-IgProtokoll herunterladen
IHC protocol for FOXO3A antibody 66428-1-IgProtokoll herunterladenl
IF protocol for FOXO3A antibody 66428-1-IgProtokoll herunterladen
IP protocol for FOXO3A antibody 66428-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IHC,IP

Autophagy

CDK9 inhibition blocks the initiation of PINK1-PRKN-mediated mitophagy by regulating the SIRT1-FOXO3-BNIP3 axis and enhances the therapeutic effects involving mitochondrial dysfunction in hepatocellular carcinoma.

Authors - Jingyue Yao
HumanIHC

Mol Ther

FOXO3A-induced LINC00926 suppresses breast tumor growth and metastasis through inhibition of PGK1-mediated Warburg effect.

Authors - Zhong Chu
humanWB

Clin Immunol

METTL14-m6A-FOXO3a axis regulates autophagy and inflammation in ankylosing spondylitis

Authors - Yuting Chen
  • KD Validated
humanWB

J Control Release

Next generation miRNA inhibition using short anti-seed PNAs encapsulated in PLGA nanoparticles.

Authors - Shipra Malik
mouseWB,IF

Am J Pathol

Tumor-derived oxidative stress triggers ovarian follicle loss in breast cancer

Authors - Yongqi Wu
humanWB

Transl Psychiatry

Psychosocial and behavioral problems of children and adolescents in the early stage of reopening schools after the COVID-19 pandemic: a national cross-sectional study in China.

Authors - Lin Wang