FOXO3A Monoklonaler Antikörper

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• KD/KO Validated

FOXO3A Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

1F12D11

Kat-Nr. : 66428-1-PBS

Synonyme

FOXO3, 1F12D11, AF6q21, AF6q21 protein, FKHRL1

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  WB analysis using 66428-1-Ig (same clone as 66428-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66428-1-Ig (FOXO3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

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  WB analysis using 66428-1-Ig (same clone as 66428-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66428-1-Ig (FOXO3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66428-1-Ig (same clone as 66428-1-PBS)

  WB result of FOXO3A antibody (66428-1-Ig, 1:12,000) with si-Control and si-FOXO3A transfected HEK293 cells. This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66428-1-Ig (same clone as 66428-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66428-1-Ig (FOXO3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

• 

  Sample test of MP51341-1

  Sample test of MP51341-1, FOXO3A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66428-1-PBS. Detection antibody: 66428-2-PBS.

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  IP experiment of HEK-293 using 66428-1-Ig (same clone as 66428-1-PBS)

  IP result of anti-FOXO3A (IP:66428-1-Ig, 4ug; Detection:66428-1-Ig 1:1000) with HEK-293 cells lysate 2200 ug. This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66428-1-Ig (same clone as 66428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66428-1-Ig (FOXO3A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66428-1-Ig (same clone as 66428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66428-1-Ig (FOXO3A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66428-1-Ig (same clone as 66428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66428-1-Ig (FOXO3A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66428-1-Ig (same clone as 66428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66428-1-Ig (FOXO3A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66428-1-Ig (same clone as 66428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66428-1-Ig (FOXO3A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 66428-1-Ig (same clone as 66428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66428-1-Ig (FOXO3A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 66428-1-Ig (same clone as 66428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66428-1-Ig (FOXO3A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66428-1-Ig (same clone as 66428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66428-1-Ig (FOXO3A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66428-1-Ig (same clone as 66428-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed HepG2 cells using 66428-1-Ig(FOXO3A antibody) at dilution of 1:50 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-mouse IgG(H+L). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51341-1

  Cytometric bead array standard curve of MP51341-1, FOXO3A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66428-1-PBS. Detection antibody: 66428-2-PBS. Standard:Ag1289. Range: 0.391-400 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66428-1-PBS bindet in WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA, Sample test FOXO3A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FOXO3A fusion protein Ag1289
• Vollständiger Name: forkhead box O3
• Berechnetes Molekulargewicht: 673 aa, 71 kDa
• Beobachtetes Molekulargewicht: 85-90 kDa
• GenBank-Zugangsnummer: BC021224
• Gene symbol: FOXO3A
• Gene ID (NCBI): 2309
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa,but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa fulllength FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after INS stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386)