FOXO4 Polyklonaler Antikörper

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• KD/KO Validated

FOXO4 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Rezensionen (1)

Kat-Nr. : 21535-1-AP

Synonyme

AFX, AFX1, forkhead box O4, Forkhead box protein O4, FOXO4, MLLT7

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Galerie der Validierungsdaten

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  WB analysis using 21535-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21535-1-AP (FOXO4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21535-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21535-1-AP (FOXO4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 21535-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21535-1-AP (FOXO4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21535-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21535-1-AP (FOXO4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, Mausherzgewebe, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

21535-1-AP bindet in WB, IHC, IF, ELISA FOXO4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FOXO4 fusion protein Ag16169
• Vollständiger Name: forkhead box O4
• Berechnetes Molekulargewicht: 505 aa, 54 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC106761
• Gene symbol: FOXO4
• Gene ID (NCBI): 4303
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor which involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1.FOXO down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias. The antibody can recognize isoform 1 and isoform Zeta of FOXO4.

Publikationen

Species	Application	Title
canine	WB	Circ Res; Suppression of Activated FOXO Transcription Factors in the Heart Prolongs Survival in a Mouse Model of Laminopathies.; Authors - Gaelle Auguste
human	WB	J Clin Invest; Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis.; Authors - Xu Xiaojie X ; KD Validated
mouse,rat	WB,IHC	Nat Commun; Damaged brain accelerates bone healing by releasing small extracellular vesicles that target osteoprogenitors.; Authors - Wei Xia
rat	WB	Redox Biol; Notoginsenoside R1-loaded mesoporous silica nanoparticles targeting the site of injury through inflammatory cells improves heart repair after myocardial infarction.; Authors - Han Li
human	IF	Mater Today Bio; Ultra-small polydopamine nanomedicine-enabled antioxidation against senescence; Authors - Jiamei Han
rat	WB	J Agric Food Chem; Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.; Authors - Shu Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

zhiping (Verified Customer) (06-04-2021)

It works pretty well for IHC.

• Applications: Immunohistochemistry
• Cell Tissue Type: human skin